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Making Calcium Competent Cells Day 1 1. ... cold centrifuge bottles. Harvest the cells by centrifugation at 3000g (~4000 rpm in the Beckman JA-10 rotor) ...
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Preheat the oven to a steady 350˚ and butter six cups in a standard-size muffin tin. ... Pour the eggs over each prepared cup and place in the oven. Bake until just ...